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    The SD BIOLINE Dengue Duo rapid test kit produced by Standard Diagnostics was used for classification of patients into primary and secondary dengue. It is a one-step immunochromatographic assay designed for the detection of both dengue virus NS1 antigen and differential IgM/IgG antibodies to dengue virus in whole blood, serum, or plasma.

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    HIV 1/2 Rapid Test Kit is a lateral flow immunochromatographic assay for the qualitative detection of antibodies (IgG, IgM, IgA) to HIV-1 (including O) or HIV-2 virus in human serum, plasma or whole blood. It is intended to be used by professionals as a screening test and an aid in the diagnosis of infection with HIV-1 or HIV-2 viruses. Any reactive specimen with the HIV 1/2 Rapid Test Kit ...

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    Field Evaluation and Impact on Clinical Management of a Rapid Diagnostic Kit That Detects Dengue NS1, IgM and IgG Anne-Claire Andries1, Veasna Duong1, Chantha Ngan2, Sivuth Ong1, Rekol Huy2, Kim Kim Sroin3, Vantha Te4, Bunthin Y1, Patrich Lorn Try3, Philippe Buchy1* 1 Institut Pasteur du Cambodge, Re´seau international des Instituts Pasteur, Phnom Penh, Cambodia, 2 National Dengue Control ...

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  • Dengue Virus Infection Diagnostics Landscape

    detect specific dengue antibodies, immunoglobulin M (IgM), immunoglobulin G (IgG) and immunoglobulin A (IgA). The choice of assay will depend both on the timing of sample collection and the purpose of the testing (10). Viraemia is generally detectable for about 4 – 5 days after the onset of fever and correlates with fever duration.

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    Background Early diagnosis of dengue virus (DENV) infection can improve clinical outcomes by ensuring close follow-up, initiating appropriate supportive therapies and raising awareness to the potential of hemorrhage or shock. Non-structural glycoprotein-1 (NS1) has proven to be a useful biomarker for early diagnosis of dengue. A number of rapid diagnostic tests (RDTs) and enzyme-linked ...

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    Analytical sensitivity of Dengue Early Rapid test for recombinant dengue NS1. The test line signal intensity was measured against a serial two-fold dilution series of DENV-1 NS1 (A), DENV-2 NS1 …

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    In vitro rapid test strips/cassettes for one step dengue NS1 antigen test or duo rapid test for both dengue NS1 antigen and IgG/IgM are available commercially from different sources (Standard Diagnostics, Inc., Korea; Biocan Diagnostics, Inc., Vancouver, BC, Canada; Diagnostic Automation and Cortez Diagnstic, Inc., Calabasas, CA).

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  • Role of NS1 antibodies in the pathogenesis of acute ...

    One such potential target is dengue non-structural protein 1 (NS1), a secreted glycoprotein used as a diagnostic marker of dengue infection, appearing early in the serum before antibodies are ...

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    Despite the increasing numbers of travel-acquired dengue, few studies have assessed virologic markers of the disease in non-endemic populations. We examined the kinetics of diagnostic markers and their associations with clinical parameters in 93 patients with travel-acquired dengue fever. Kinetics analyses suggested a longer average duration for viremia (9 days, CI95%: 8–10) and non ...

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    Dengue fever is a mosquito-borne viral infection and is commonly found in many tropical and sub-tropical countries. Global incidences have increased dramatically over the last decade and it was ...

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    Dengue fever, a mosquito-borne viral disease has become a major worldwide public health problem with a dramatic expansion in recent years. Cultivation process for production of recombinant dengue virus type 4 envelope domain III (rDen 4 EDIII) protein in Escherichia coli was developed for its diagnostic use as well as for further studies in immunoprophylaxis.

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    Expression of dengue-2 virus NS1 protein in Nicotiana tabacum plants for development of dengue immunodiagnostic kits. Dengue is one of the most important diseases caused by arboviruses in the world. A significant increase in its geographical distribution has been noticed over the last 20 years, with continuous transmission of several serotypes and emergence of the hemorrhagic fever in areas ...

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    Dengue fever, caused by one of the four dengue virus serotypes (DENV1-DENV4), is a mosquito-borne disease of public health importance due to its increasing burden worldwide [].With 390 million estimated infections and 10,000 deaths per year, dengue is the predominant mosquito-borne virus in humans [].The threat of a possible dengue outbreak due to the introduction of dengue virus by viremic ...

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  • Dengue Virus

    It is a simple, rapid test that requires very little sophisticated equipment (Kapoor et al., 1995)). Anti-dengue IgM antibody develops a little faster than IgG antibody, MAC-ELISA shows a sensitivity and specificity of 90% and 98% in samples collected after 5 days of fever respectively (PAHO, 1994).

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    Dengue is one of the major public health concerns in the world. Since all the four serotypes are actively circulating in Mexico, there is a need to develop an efficient diagnosis system to improve case management of the patients. There exist few studies evaluating the use of the NS3 protein as a protective antigen against dengue virus (DENV). In this paper we show the expression of a ...

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